MOTS-C — Mitochondrial Cycle
mots-c is a mitochondria-derived peptide that activates ampk — the cell's energy sensor — supporting metabolic health and exercise capacity as a daily cycle.
Why this protocol
mots-c is encoded inside mitochondrial DNA and acts as a signal back to the rest of the cell. Its best-characterized lever is activating ampk, the energy sensor that switches on fat-burning and mitochondrial work when energy runs low — which is why it is described as an 'exercise mimetic.'
The longevity and metabolic rationale follows from that: better insulin sensitivity, improved metabolic flexibility, and endurance support. It is run as a daily (or near-daily) mono cycle.
Convention drawn from the MOTS-C page's cited evidence — human data are early, so the dose and schedule are practitioner/community convention extrapolated from mechanism, not a trial-proven regimen. No therapeutic claims.
The regimen
MOTS-c
Mitochondrial / AMPK- Dose
- 500–1,000 mcg
- Schedule
- Once daily (or near-daily) · subcutaneous
- Timing
- Often dosed before exercise (exercise-mimetic rationale)
Reconstitution calculator
Reconstitution calculator
Calculated for a 1 mL U-100 insulin syringe (100 units/mL).
Units per dose
20
Draw to this mark on a U-100 syringe
- Volume per dose
- 0.2 mL
- Doses per vial
- 10
- Concentration
- 5 mg/mL
One vial lasts
- Daily
- 10 days
- Every other day
- 20 days
- 5×/week
- 14 days
Research use only. Not for human consumption. Outputs are reference values based on research literature — verify all measurements independently.
Cycle & schedule
Cycle (8–12 weeks)
MOTS-C 500–1,000 mcg once daily, roughly aligning the dose with training where exercise capacity is the goal.
Washout (2–4 weeks)
A 2–4-week break before repeating; recheck any metabolic markers (e.g. fasting insulin) you are tracking.
Studies behind this protocol
This protocol composes the cited evidence on each peptide's page — see the full bibliography on: MOTS-c.
Research use only · Not medical advice · Updated 2026-06-01