NAD+ for a cellular energy cycle
NAD+ replenishes the coenzyme that powers energy production and sirtuin-driven repair. Both decline with age. This is a daily subcutaneous cycle to top them back up.
4–8 weeks + 2–4-week washout
Why this protocol
NAD+ is a coenzyme every cell uses to turn fuel into energy and to run DNA repair and the sirtuin enzymes tied to healthy aging. Its levels fall with age, and replenishing it is the whole longevity rationale here. Clinics typically deliver it by IV; the subcutaneous route here is the at-home community approach to the same goal.
It is a mono protocol, a single coenzyme on a daily schedule. The main practical issue is the flushing/urge sensation a fast dose can cause, which is why it is injected slowly and titrated up rather than started high.
The regimen
NAD+
Coenzyme repletion- Dose
- 50–100 mg
- Schedule
- Once daily · subcutaneous
- Timing
- Inject slowly; flushing is dose-related, so titrate up
Reconstitution calculator
Reconstitution calculator
Calculated for a 1 mL U-100 insulin syringe (100 units/mL).
Units per dose
50 units
Draw this many units on a U-100 syringe
Each unit holds 1,000 mcg
- Volume per dose
- 0.5 mL
- Doses per vial
- 2
- Concentration
- 100 mg/mL
One vial lasts
- Daily
- 2 days
- Every other day
- 4 days
- 5×/week
- 2 days
- Large draw (50 units). That's normal for mg doses. To check it, multiply the units by what each unit holds. You should get your dose.
Research use only. Not for human consumption. Outputs are reference values based on research literature. Verify all measurements independently.
Cycle & schedule
- Cycle (4–8 weeks)NAD+ 50–100 mg once daily subcutaneously, starting at the low end and injecting slowly to limit flushing.
- Washout (2–4 weeks)Take 2–4 weeks off; the break is when to recheck any metabolic or energy markers you are tracking.
Studies behind this protocol
This protocol composes the cited evidence on each peptide's page. See the full bibliography on: NAD+.
Research use only · Not medical advice · Updated 2026-09-24