NAD+ — Cellular Energy Cycle
nad-plus replenishes the coenzyme that powers energy production and sirtuin-driven repair — both of which decline with age — as a daily subcutaneous cycle.
Why this protocol
nad-plus is a coenzyme every cell uses to turn fuel into energy and to run DNA-repair and the sirtuin enzymes tied to healthy aging. Its levels fall with age, and the longevity rationale is simple: top the pool back up. Clinics typically deliver it by IV; the subcutaneous route here is the at-home community approach to the same goal.
It is a mono protocol — a single coenzyme on a daily schedule. The main practical issue is the flushing/urge sensation a fast dose can cause, which is why it is injected slowly and titrated up rather than started high.
Convention drawn from the NAD+ page's cited evidence — subcutaneous self-dosing is the community adaptation of clinic IV protocols, so the dose and schedule are convention rather than a trial-proven regimen. No therapeutic claims.
The regimen
NAD+
Coenzyme repletion- Dose
- 50–100 mg
- Schedule
- Once daily · subcutaneous
- Timing
- Inject slowly; flushing is dose-related, so titrate up
Reconstitution calculator
Reconstitution calculator
Calculated for a 1 mL U-100 insulin syringe (100 units/mL).
Units per dose
50
Draw to this mark on a U-100 syringe
- Volume per dose
- 0.5 mL
- Doses per vial
- 2
- Concentration
- 100 mg/mL
One vial lasts
- Daily
- 2 days
- Every other day
- 4 days
- 5×/week
- 2 days
- Large draw (50 units). Double-check the vial size and dose — a mcg/mg mix-up produces values like this.
Research use only. Not for human consumption. Outputs are reference values based on research literature — verify all measurements independently.
Cycle & schedule
Cycle (4–8 weeks)
NAD+ 50–100 mg once daily subcutaneously, starting at the low end and injecting slowly to limit flushing.
Washout (2–4 weeks)
Take 2–4 weeks off; the break is when to recheck any metabolic or energy markers you are tracking.
Studies behind this protocol
This protocol composes the cited evidence on each peptide's page — see the full bibliography on: NAD+.
Research use only · Not medical advice · Updated 2026-06-01