NAD+ + Glutathione for cellular maintenance
NAD+ powers energy production and repair. Glutathione mops up the oxidative cost of running hot. Together they produce and protect, a complementary cellular-maintenance pair.
NAD+ 4–8-week blocks · Glutathione ongoing 2–3×/week
Why this protocol
NAD+ is the coenzyme that drives mitochondrial energy production, sirtuin activity, and DNA repair. It raises the cell's metabolic output. Running hotter, though, generates oxidative byproducts.
Glutathione is the master intracellular antioxidant that neutralizes exactly that oxidative stress. So the pair is genuinely complementary. NAD+ turns the engine up, glutathione handles the exhaust. They pull different levers, not two antioxidants or two energy boosters.
The regimen
NAD+
Energy / sirtuins- Dose
- 50–100 mg
- Schedule
- Once daily · subcutaneous
- Timing
- Inject slowly; flushing is dose-related, so titrate up
Reconstitution calculator
Reconstitution calculator
Calculated for a 1 mL U-100 insulin syringe (100 units/mL).
Units per dose
50 units
Draw this many units on a U-100 syringe
Each unit holds 1,000 mcg
- Volume per dose
- 0.5 mL
- Doses per vial
- 2
- Concentration
- 100 mg/mL
One vial lasts
- Daily
- 2 days
- Every other day
- 4 days
- 5×/week
- 2 days
- Large draw (50 units). That's normal for mg doses. To check it, multiply the units by what each unit holds. You should get your dose.
Research use only. Not for human consumption. Outputs are reference values based on research literature. Verify all measurements independently.
Glutathione
Antioxidant defense- Dose
- 400 mg per session
- Schedule
- 2–3× per week · subcutaneous
- Timing
- Any time of day
Reconstitution calculator
Reconstitution calculator
Calculated for a 1 mL U-100 insulin syringe (100 units/mL).
Units per dose
400 units
That's 4 mL, more than a 1 mL syringe holds.
Each unit holds 1,000 mcg
- Volume per dose
- 4 mL
- Doses per vial
- 1
- Concentration
- 100 mg/mL
One vial lasts
- Daily
- 1 day
- Every other day
- 3 days
- 5×/week
- 2 days
- Dose volume (4 mL) exceeds the 1 mL syringe capacity. Use a larger syringe or split the draw.
Research use only. Not for human consumption. Outputs are reference values based on research literature. Verify all measurements independently.
Cycle & schedule
- Run togetherNAD+ 50–100 mg once daily (start low, inject slowly) in 4–8-week blocks; Glutathione 400 mg 2–3× per week on an ongoing basis alongside it.
- NAD+ washout (2–4 weeks)Pause NAD+ for 2–4 weeks between blocks; glutathione can continue as maintenance. Recheck any markers during the break.
Studies behind this protocol
This protocol composes the cited evidence on each peptide's page. See the full bibliography on: NAD+, Glutathione.
Research use only · Not medical advice · Updated 2026-09-24