NAD+ + Glutathione — Cellular Maintenance
nad-plus powers energy production and repair while glutathione mops up the oxidative cost of running hot — produce and protect, a complementary cellular-maintenance pair.
Why this protocol
nad-plus is the coenzyme that drives mitochondrial energy production, sirtuin activity, and DNA repair — it raises the cell's metabolic output. Running hotter, though, generates oxidative byproducts.
glutathione is the master intracellular antioxidant that neutralizes exactly that oxidative stress. So the pair is genuinely complementary: NAD+ turns the engine up, glutathione handles the exhaust — different levers, not two antioxidants or two energy boosters.
Community / practitioner convention assembled from each peptide's cited evidence — not a trial-proven combined regimen. Doses match each peptide's own page; clinics often deliver both intravenously, so the subcutaneous schedule is community convention.
The regimen
NAD+
Energy / sirtuins- Dose
- 50–100 mg
- Schedule
- Once daily · subcutaneous
- Timing
- Inject slowly; flushing is dose-related, so titrate up
Reconstitution calculator
Reconstitution calculator
Calculated for a 1 mL U-100 insulin syringe (100 units/mL).
Units per dose
50
Draw to this mark on a U-100 syringe
- Volume per dose
- 0.5 mL
- Doses per vial
- 2
- Concentration
- 100 mg/mL
One vial lasts
- Daily
- 2 days
- Every other day
- 4 days
- 5×/week
- 2 days
- Large draw (50 units). Double-check the vial size and dose — a mcg/mg mix-up produces values like this.
Research use only. Not for human consumption. Outputs are reference values based on research literature — verify all measurements independently.
Glutathione
Antioxidant defense- Dose
- 400 mg per session
- Schedule
- 2–3× per week · subcutaneous
- Timing
- Any time of day
Reconstitution calculator
Reconstitution calculator
Calculated for a 1 mL U-100 insulin syringe (100 units/mL).
Units per dose
600
Draw to this mark on a U-100 syringe
- Volume per dose
- 6 mL
- Doses per vial
- 1
- Concentration
- 100 mg/mL
One vial lasts
- Daily
- 1 days
- Every other day
- 2 days
- 5×/week
- 1 days
- Dose volume (6 mL) exceeds the 1 mL syringe capacity. Use a larger syringe or split the draw.
- Large draw (600 units). Double-check the vial size and dose — a mcg/mg mix-up produces values like this.
Research use only. Not for human consumption. Outputs are reference values based on research literature — verify all measurements independently.
Cycle & schedule
Run together
NAD+ 50–100 mg once daily (start low, inject slowly) in 4–8-week blocks; Glutathione 400 mg 2–3× per week on an ongoing basis alongside it.
NAD+ washout (2–4 weeks)
Pause NAD+ for 2–4 weeks between blocks; glutathione can continue as maintenance. Recheck any markers during the break.
Studies behind this protocol
This protocol composes the cited evidence on each peptide's page — see the full bibliography on: NAD+, Glutathione.
Research use only · Not medical advice · Updated 2026-06-01